An HPLC demonstration shows how to separate, identify, and quantify liquid components using high pressure to push a sample through a column, resulting in peaks on a graph representing different compounds, involving mobile phase prep (degassing/filtering), method setup (gradient/isocratic), sample injection, and data analysis. Key steps include filtering and degassing solvents, setting flow rates and wavelengths, injecting a filtered sample via auto-sampler or syringe, monitoring detector signals for peaks over time, and post-run analysis to identify fractions.
Great for you
A variety of solutions can be obtained from HPLC Engineer’s to assist maintain the optimal operation of your system. For your system or issue detector, pick from advance exchange, repair, or upgrade options as well as spare parts.
Make it happen
Instruments must be installed, operated and maintained within design specifications, while processes must be shown to be reliable and to provide assurance that consistent product quality and integrity is maintained.
Our validation procedures are regularly updated to match the latest H/W and S/W updates of our current instrument range.
